Acarological Society of Iran Persian Journal of Acarology 2251-8169 8 4 2026 01 01 The Iranian Hyalomma(Acari: Ixodidae) with molecular evidencesto understand taxonomic statusof species complexes 291 308 239324 10.22073/pja.v8i4.49892 EN Asadollah Hosseini Chegeni Department of Plant Protection,Pol-e Dokhtar Higher Education Center, Lorestan University, Pol-e Dokhtar, Iran; E-mail: hosseinichegeni@gmail.com Reza Hosseini Zakkyeh Telmadarraiy Department of Medical Entomology and Vector Control, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; E-mail:telmadarraiy@tums.ac.ir Mohammad Abdigoudarzi Razi Vaccine and Serum Research Institute, Department of Parasitology, Reference Laboratory of Ticks and Tick Borne Diseases, Karaj, Iran; E-mail: m.abdi@rvsri.ac.ir Journal Article The identification of Hyalomma is a challenging issue in the systematics of ixodid ticks. Here, we examined 960 adult males of Hyalomma tick from 10 provinces of Iran using morphological and molecular methods. PCR was carried out on 60 samples to amplify an ITS2 fragment of nuclear and a COI fragment of mitochondrial genomes. Nine species, namely H. aegyptium, H. anatolicum, H. asiaticum, H. scupense, H. dromedarii, H. excavatum, H. marginatum, H. rufipes and H. schulzei were identified. The validity of H. rufipes and H. excavatum can be challenged. We concluded that these species should be regarded as H. marginatum and H. anatolicum complexes, respectively. Furthermore, the taxonomic status of the two closely related H. anatolicum and H. asiaticum was confirmed as distinct species.

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