Persian Journal of Acarology

Persian Journal of Acarology

Susceptibility of the sweet pepper (Capsicum annuum L.) to the infestation of Tetranychus urticae (Acari: Tetranychidae) and the different insect pests under greenhouse conditions in Ismailia, Egypt

Document Type : Original Article

Authors
1 Department of Vegetable and Ornamental Plant Mites, Plant Protection Research Institute, Agricultural Research Center (ARC), Cairo
2 Vegetable Pests, Ornamental, Medicinal and Aromatic Plants Department, Plant Protection Research Institute, Agricultural Research Center, Cairo, Egypt
Abstract
The sweet pepper, an important export ‎crop in Egypt, has been recently invaded by the two-spotted spider mite (TSSM) in parallel with other insect pests that have the potential to reduce its yield. This study aimed to determine the susceptibility of two sweet pepper cultivars (cv. Mazurka and Cannon) to TSSM infestation. In addition, it aimed to investigate how the cultivar as well as the growing season, might affect the population fluctuations of the pests along with the host plant resistance. Also, enzymatic and non-enzymatic antioxidant activities were determined to distinguish between the two cultivars after natural infestation. The TSSM was identified as the primary pest that invaded both cultivars during the two examined growing seasons. In addition to the TSSM, three insect pests (Bemisia tabaci, Frankliniella occidentalis, and Myzus persicae) and four predators (Neoseiulus cucumeris, Phytoseiulus persimilis, Scolothrips longicornis, and Orius laevigatus) were identified. The cv. Cannon was found to have the highest density of TSSM, B. tabaci, and M. persicae than cv. Mazurka demonstrated the highest density of P. persimilis and O. laevigatus. Our findings also revealed that the winter-spring season was highly significant in antioxidant enzyme activities than the summer-fall season. Due to the high chlorophyll and phenol contents, and antioxidant enzyme activity in its leaves, the Mazurka cultivar was more resistant to TSSM and other insect pest infestations. The population density of the two-spotted spider mite and the associated pests change based on season and/or cultivar.
Keywords

Aebi, H. (1984) Catalase in vitro. In: Parker, L. (Ed.), Methods in Enzymology. Academic Press, New York, pp. 121–126. DOI: 10.1016/S0076-6879(84)05016-3
Afifi, A.A.M., El-Laithy, A.Y.M., Shehata, S.A. & El-Saiedy, ES.M.A. (2010) Resistance of strawberry plants against the two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae). In: Sabelis, M.W. & Bruin, J. (Eds.), Trends in Acarology. Proceedings of the 12th International Congress, Springer, Dordrecht, Amsterdam, The Netherlands, pp. 505–507. DOI: 10.1007/978-90-481-9837-5_85
Ali, F.S., Afifi, A.M., El-Saiedy, E.M. & Ahmed, M.M. (2015) Effect of phytochemical components, morphological and histological leaf structure of five tomato hybrids on Tetranychus urticae Koch Infestation. Acarines, 9(1): 23–30. DOI: 10.21608/AJESA.2015.163978
Arimura, G.I., Matsui, K. & Takabayashi, J. (2009) Chemical and molecular ecology of herbivore-induced plant volatiles: proximate factors and their ultimate functions. Plant and Cell Physiology, 50(5): 911–923. DOI:10.1093/pcp/pcp030
Arnon, D.I. (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology, 24: 1–15. DOI: 10.1104/pp.24.1.1
Bakr, A.A., Rezk, H.A., Saleh, S.M. & El-Morshedy, N.H. (2020) Significance of foliar sprayed salicylic acid in kidney bean resistance against Tetranychus urticae (Trombidiformes: Tetranychidae) attack. Persian Journal of Acarology, 9(2): 193–205. DOI: 10.22073/pja.v9i2.59408
Bale, J.S., Masters, G.J., Hodkinson, I.D., Awmack, C., Bezemer, T.M., Brown, V.K., Butterfield, J., Buse, A., Coulson, J.C., Farrar, J. & Good, J.E. (2002) Herbivory in global climate change research: direct effects of rising temperature on insect herbivores. Global Change Biology, 8(1): 1–16. DOI: 10.1046/j.1365-2486.2002.00451.x
Bradford, M.M. (I976) A rapid and sensitive method for the ‎quantitation of microgram quantities of proteins utilizing the principle of ‎protein-dye binding. Analytical Biochemistry, 72: 248–254. DOI: 10.1016/0003-2697(76)90527-3
Calvo, F.J., Bolckmans, K. & Belda, J.E. (2012) Biological control-based IPM in sweet pepper greenhouses using Amblyseius swirskii (Acari: Phytoseiidae). Biocontrol Science and Techno-logy, 22(12): 1398–1416. DOI: 10.1080/09583157.2012.731494
Çobanoğlu, S. & Kumral, N.A. (2016) The biodiversity, density and population trend of mites (Acari) on Capsicum annuum L. in temperate and semi-arid zones of Turkey. Systematic and Applied Acarology, 21(7): 907–918. DOI: 10.11158/saa.21.7.5
Constabel, C.P., Yip, L., Patton, J.J. & Christopher, M.E. (2000) Polyphenol oxidase from hybrid poplar. Cloning and expression in response to wounding and herbivory. Plant Physiology, 124: 285–296. DOI: 10.1104/pp.124.1.285
El-Laithy, A.Y.M., Elseedy, E.M.A., El-Kholi, M.Y., Abou-Ellela M.M. & Svobodová, Z. (2013) Population dynamics of major insect and mite pests and control on sweet pepper grown in net house in Egypt. IOBC/WPRS Bulletin, 93: 31–38.
El-Saiedy, E.M., Ali, F.S., Hassan, M.F. & Hassan, A.S. (2013) Susceptibility of eight sweet pepper cultivars to infestation with Tetranychus urticae, Aphis gossypii and Thrips tabaci. Acarines, 7(2): 77–83. DOI: 10.21608/ajesa.2013.163786
El-Sayed, S.F., Hassan, H.A. & Mahmoud, S.O. (2015) Effect of some soilless culture techniques on sweet pepper growth, production, leaves chemical contents and water consumption under greenhouse conditions. Middle East Journal of Agriculture Research, 4(4): 682–691.
Fahim, S.F., Momen, F.M. & El-Saiedy, E.S.M. (2020) Life table parameters of Tetranychus urticae (Trombidiformes: Tetranychidae) on four strawberry cultivars. Persian Journal of Acarology, 9(1): 43–56. DOI: 10.22073/pja.v9i1.54771
Gallardo A., Vásquez, C., Morales, J. & Gallardo, J. (2005) Biology and natural enemies of Tetranychus urticae in sweet pepper. Manejo Integrado de Plagas y Agroecología, 74: 34–40.
Golan, K., Sempruch, C., Górska-Drabik, E., Czerniewicz, P., Łagowska, B., Kot, I., Kmieć, K., Magierowicz, K. & Leszczyński, B. (2017) Accumulation of amino acids and phenolic compounds in biochemical plant responses to feeding of two different herbivorous arthropod pests. Arthropod-Plant Interactions, 11: 675–682. DOI: 10.1007/s11829-017-9522-8
Gotoh, T. & Gomi, K. (2000) Population dynamics of Tetranychus kanzawai (Acari: Tetranychidae) on hydrangea. Experimental and Applied Acarology, 24: 337–350. DOI: 10.1023/A:1006428503 702
Habig, W.H., Pabst, M.J. & Jakoby, W.B. (1974) Glutathione-S-transferase: the first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249: 7130–7139. DOI: 10.1016/ S0021-9258(19)42083-8
Helmi, A. & Rashwan, R. (2015) Susceptibility of some solanaceous plant cultivars to sap-sucking insects infestation and their associated natural enemies. Journal of Plant Protection and Pathology, 6(5): 763–781. DOI: 10.21608/jppp.2015.74501
Hildebrand, D.F., Rodrigues, J.G., Brown, G.C., Luu, K.T. & Volden, C.S. (1986) Peroxidase responses of leaves in two soybean genotypes injured by two-spotted spider mites (Acari: Tetranychidae). Journal of Economic Entomology, 79: 915–921. DOI: 10.1093/jee/79.6.1459
Ishaaya, I. (1971) Observations on the phenoloxidase system in the armored scales Aonidiella aurantii and Chrysomphalus aonidum. Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 39(4): 935–943. DOI: 10.1016/0305-0491(71)90117-9
Johnson, K.S. & Felton, G.W. (2001) Plant phenolics as dietary antioxidants for herbivorous insects: a test with genetically modified tobacco. Journal of Chemical Ecology, 27: 2579–2597. DOI: 10. 1023/A:1013691802028
Kähkönen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T.S. & Heinonen, M. (1999) Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural Food Chemistry, 47(10): 3954–3962. DOI: 10.1021/jf990146l
Kielkiewicz, M. (2004) Induced resistance of tomato (Lycopersicon esculentum Mill.) in response to the carmine spider mite (Tetranychus cinnabarinus Boisduval) feeding - A case study. Progress in Plant Protection, 44(1): 138–146 (In Polish).
Kettles, G.J. & Kaloshian, I. (2016) The potato aphid salivary effector Me47 is a glutathione-S-transferase involved in modifying plant responses to aphid infestation. Frontier in Plant Science, 7: 1142. DOI: 10.3389/fpls.2016.01142
Kim, S., Lee, J.K., Song, Y.J., Kang, S.C., Kim, B., Choi, I.J. & Lee, D.H. (2018) Evaluating natural compounds as potential insecticides against three economically important pests, Bemisia tabaci (Hemiptera: Aleyrodidae), Frankliniella occidentalis (Thysanoptera: Thripidae), and Myzus persicae (Hemiptera: Aphididae), on greenhouse sweet peppers. Applied Biological Chemistry, 61(3): 313–323. DOI: 10.1007/s13765-018-0362-8
Kitashima, Y. & Gotoh, T. (2003) Population dynamics of Panonychus osmanthi (Acari: Tetranych-idae) on two Osmanthus species. Experimental and Applied Acarology, 29: 227–24. DOI: 10.102 3/A:1025896214163
Kong, H.L., Lü, M., Wu, L. & Zhu, S.D. (2014) Effects of Bemisia tabaci damage on the protective enzyme activity and the secondary metabolite content of leaves in different pepper varieties. Chinese Journal of Applied Entomology, 51(6): 1553–1560. DOI: 10.7679/j.issn.2095-1353.201 4.181
Langcake, P. & Wickins, S.G. (1975) Studies on the action of the dichlorocyclopropanes on the host-parasite relationship in the rice blast disease. Physiological Plant Pathology, 7(2): 113–126. DOI: 10.1016/0048-4059(75)90002-8
Li, L. & Steffens, J.C. (2002) Overexpression of polyphenol oxidase in transgenic tomato plants results in enhanced bacterial disease resistance. Planta, 215: 239–247. DOI: 10.1007/s00425-002-0750-4
Li, Q., He, Y., Yang, Q., Jiang, C., Wang, H. & Jiang, S. (2015) Relationships between several secondary substances in pepper varieties and the resistance to broad mite Polyphagotarsonemus latus (Banks). Acta Phytophylacica Sinica, 42: 283–288. DOI: 10.13802/j.cnki.zwbhxb.2015.02. 021
Lu, F., Chen, Z., Lu, H., Liang, X., Zhang, H., Li, Q., Chen, Q., Huang, H., Hua, Y. & Tian, W. (2016) Effects of resistant and susceptible rubber germplasm on development, reproduction and protective enzyme activities of Eotetranychus sexmaculatus (Acari: Tetranychidae). Experimental and Applied Acarology, 69: 427–443. DOI: 10.1007/s10493-016-0049-y
Maklad, A.M., Yassin, S.A. & El-Ghafar, A. (2014) Influence of certain climatic factors on some major pepper pests under Egyptian conditions. Egyptian Academic Journal of Biological Sciences: A. Entomology, 7(1): 31–37. DOI: 10.21608/eajbsa.2014.13199
Mustafa, A.A. & Al Mallah, N.T. (2021) Seasonal activity and sensitivity of some tomato cultivars to Tetranychus urticae Koch. NTU Journal of Agricultural and Veterinary Sciences, 1(1): 29–37. DOI: 10.56286/ntujavs.v1i1.41
Pappas, M.L., Broekgaarden, C., Broufas, G.D., Kant, M.R., Messelink, G.J., Steppuhn, A., Wäckers, F. & Van Dam, N.M. (2017) Induced plant defences in biological control of arthropod pests: a double‐edged sword. Pest Management Science, 73(9): 1780–1788. DOI: 10.1002/ps.4587
Park, Y. & Lee, J. (2002) Leaf cell and tissue damage of cucumber caused by two-spotted spider mite (Acari: Tetranychidae). Journal of Economic Entomology, 95(1): 952–957. DOI: 10.1093/jee/95. 5.952
Patel, N.B., Bhatt, N.A. & Patel, C.C. (2020) Effect of weather parameters on incidence of brinjal mite, Tetranychus urticae Koch and its predatory mite, Amblyseius alstoniae Gupta. Journal of Pharmacognosy and Phytochemistry, 9(4): 3095–3099.
Rahmani, H., Fathipour, Y. & Kamali, K. (2010) Spatial distribution and seasonal activity of Panonychus ulmi (Acari: Tetranychidae) and its predator Zetzellia mali (Acari: Stigmaeidae) in apple orchards of Zanjan, Iran. Journal of Agricultural Science and Technology, 12(2): 155–165.
Romeih, A.M., El-Saiedy, E.A. & Sholla, S.E. (2013) Study the population dynamics of two spotted spider mite Tetranychus urticae Koch infesting two Faba bean cultivars. Life Science Journal, 10(3): 1328–1333.
Sereme, A., Dabire, C., Koala, M., Somda, M.K. & Traore, A.S. (2016) Influence of organic and mineral fertilizers on the antioxidants and total phenolic compounds level in tomato (Solanum lycopersicum) var. Mongal F1. Journal of Experimental Biology and Agricultural Sciences, 4(4): 414–420. DOI: 10.18006/2016.4(4).414.420
Sharma, H.C. & Ortiz, R. (2002) Host plant resistance to insects: an eco-friendly approach for pest management and environment conservation. Journal of Environmental Biology, 23(2): 111–135.
Singleton, V.L. & Rossi, J.A. (1965) Colorimetry of total phenolics with Phosphomolybdic-Phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16(3): 144–158.
Soffan, A., Alghamdi, S. & Aldawood, A. (2014) Peroxidase and polyphenol oxidase activity in moderate resistant and susceptible Vicia faba induced by Aphis craccivora (Hemiptera: Aphididae) infestation. Journal of Insect Science, 14(1): 285. DOI: 10.1093/jisesa/ieu147
Ssemwogerere, C., Ochwo-Ssemakula, M.N., Kovach, J., Kyamanywa, S. & Karungi, J. (2013) Species composition and occurrence of thrips on tomato and pepper as influenced by farmers’ management practices in Uganda. Journal of Plant Protection Research, 53(2): 153–164. DOI: 10.2478/jppr-2013-0024
Steinite, I. & Ievinsh, G. (2002) Wound-induced responses in leaves of strawberry cultivar differing in susceptibility to spider mite. Journal of Plant Physiology, 159: 491–497. DOI: 10.1078/0176-1617-00683
Stout, M.J., Workman, K.V., Bostock, R.M. & Duffey, S.S. (1997) Specificity of induced resistance in the tomato Lycopersicon esculentum. Oecologia, 113: 74–81. DOI: 10.1007/s004420050355
Tehri, K., Gulati, R., Geroh, M. & Madan, S. (2014) Effect of two-spotted spider mite infestation on some biochemical parameters of cucumber leaves. Annals of Biology, 30(4): 686–690.
Wang, S., Zhang, Y., Qin, Y. & Zhu, G. (2010) Population dynamics of carmine spider mite, Tetranychus cinnabarinus, under intensive culture in Beijing. Chinese Bulletin of Entomology, 47(1): 72–75.
Wettstein, V.D. (1957) Chlorophyll-letale and der submikroskopishe Formweschsel der Plastiden. Experimental Cell Research, 12(3): 427–506. DOI: 10.1016/0014-4827(57)90165-9
Ximénez-Embún, M.G., Ortego, F. & Castañera, P. (2016) Drought-stressed tomato plants trigger bottom–up effects on the invasive Tetranychus evansi. PloS ONE, 11(1): e0145275. DOI: 10.1371/journal.pone.0145275
Yigezu, G., Wakgari, M. & Goftishu, M. (2019) Assessment of two-spotted spider mite (Tetranychus urticae) on potato (Solanum tuberosum L.) in Eastern Hararghe, Ethiopia. International Journal of Environment Agriculture and Biotechnology, 4(6): 1862–1873. DOI: 10.22161/ijeab.46.37
Zayed, M.S., Hassanein, M.K., Esa, N.H. & Abdallah, M.F. (2013) Productivity of pepper crop (Capsicum annuum L.) as affected by organic fertilizer, soil solarization, and endomycorrhizae. Annals of Agricultural Sciences, 58(2): 131–137. DOI: 10.1016/j.aoas.2013.07.011
Zhang, Y., Bouwmeester, H.J. & Kappers, I.F. (2020) Combined transcriptome and metabolome analysis identifies defence responses in spider mite-infested pepper (Capsicum annuum). Journal of Experimental Botany, 71(1): 330–343. DOI: 10.1093/jxb/erz422