Varroa destructor Anderson & Truman is the most serious pest of honey bee colonies all over the world. Its specimens were assessed by morphometric and geometric morphometric methods using six morphometric traits including body length and width, length and width of the right metapodal shield, length of epigynal and the anal shield for classical morphometrics and six landmarks in ventral surface of mite's body, in seven provinces including Ardabil, Markazi, Qom, Qazvin, Alborz, North Khorasan, and Semnan. Twenty and ten individuals were used for morphometric and geometric morphometric studies, respectively. The ventral surface of each mite slide was prepared and used Digimizer and Tps package software for morphometric and geometric morphometric measurements, respectively. Data were analyzed with Past software. Furthermore, principal component analysis (PCA) was used for both two methods. In addition, Cluster analysis was used to categorize specimens of provinces. The results demonstrated significantly morphometric and geometric morphometric (or shape and size) differences among the specimens of provinces. Qazvin and Ardabil were located in one group with the PCA and Cluster analysis in the classical morphometric method and Alborz mite's dimensions showed a significant difference in morphometric measurements with other provinces. However, in the geometric morphometric method, Ardabil, Markazi, North Khorasan, and Semnan specimens were located in another group. The difference in distribution can be related to geographical factors and methods of measurement.